Shiga Toxin Detection Methods : A Short Review

The Shiga toxins comprise a family of related protein toxins secreted by certain types of bacteria. Shigella dysenteriae, some strain of Escherichia coli and other bacterias can express toxins which caused serious complication during the infection. Shiga toxin and the closely related Shiga-like toxins represent a group of very similar cytotoxins that may play an important role in diarrheal disease and hemolytic-uremic syndrome. The outbreaks caused by this toxin raised serious public health crisis and caused economic losses. These toxins have the same biologic activities and according to recent studies also share the same binding receptor, globotriosyl ceramide (Gb3). Rapid detection of food contamination is therefore relevant for the containment of food-borne pathogens. The conventional methods to detect pathogens, such as microbiological and biochemical identification are time-consuming and laborious. The immunological or nucleic acid-based techniques require extensive sample preparation and are not amenable to miniaturization for on-site detection. In the present are necessary of techniques of rapid identification, simple and sensitive which can be employed in the countryside with minimally-sophisticated instrumentation. Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications.Comment: 16 pages, 2 figure

Room temperature ferromagnetic-like behavior in Mn-implanted and post-annealed InAs layers deposited by Molecular Beam Epitaxy

We report on the magnetic and structural properties of Ar and Mn implanted InAs epitaxial films grown on GaAs (100) by Molecular Beam Epitaxy (MBE) and the effect of Rapid Thermal Annealing (RTA) for 30 seconds at 750C. Channeling Particle Induced X- ray Emission (PIXE) experiments reveal that after Mn implantation almost all Mn atoms are subsbtitutional in the In-site of the InAs lattice, like in a diluted magnetic semiconductor (DMS). All of these samples show diamagnetic behavior. But, after RTA treatment the Mn-InAs films exhibit room-temperature magnetism. According to PIXE measurements the Mn atoms are no longer substitutional. When the same set of experiments were performed with As as implantation ion all of the layers present diamagnetism without exception. This indicates that the appearance of room-temperature ferromagnetic-like behavior in the Mn-InAs-RTA layer is not related to lattice disorder produce during implantation, but to a Mn reaction produced after a short thermal treatment. X-ray diffraction patterns (XRD) and Rutherford Back Scattering (RBS) measurements evidence the segregation of an oxygen deficient-MnO2 phase (nominally MnO1.94) in the Mn-InAs-RTA epitaxial layers which might be on the origin of room temperature ferromagnetic-like response observed.Comment: 16 pages, 5 figures. Acepted in J. Appl. Phy

InAs/InP single quantum wire formation and emission at 1.5 microns

Isolated InAs/InP self-assembled quantum wires have been grown using in situ accumulated stress measurements to adjust the optimal InAs thickness. Atomic force microscopy imaging shows highly asymmetric nanostructures with average length exceeding more than ten times their width. High resolution optical investigation of as-grown samples reveals strong photoluminescence from individual quantum wires at 1.5 microns. Additional sharp features are related to monolayer fluctuations of the two dimensional InAs layer present during the early stages of the quantum wire self-assembling process.Comment: 4 pages and 3 figures submitted to Applied Physics Letter

Magnetotunneling in a Two-Dimensional Electron-Hole System Near Equilibrium

We have measured the zero-bias differential tunneling conductance of InAs/AlSb/GaS b/AlSb/InAs heterostructures at low temperatures (1.7K < T < 60K) and unde r a magnetic field at various angles with the heterostructure's interfaces. Shubni kov-de Haas oscillations in the magnetoconductance reveal the two-dimensional (2D) character of the electrons accumulated at the InAs interfaces and yield their num ber in each of them. The temperature dependence of the oscillations suggests the f ormation of a field-induced energy gap at the Fermi level, similar to that observe d before in simpler 2D-2D tunneling systems. A calculation of the magnetoconductan ce that considers different 2D densities in the two InAs electrodes agrees with th e main observations, but fails to explain features that might be related to the pr esence of 2D holes in the GaSb region.Comment: 4 papes, 3 eps figures. Submit to Phys. Rev.

Viscoelastic model for the dynamic structure of binary systems

This paper presents the viscoelastic model for the Ashcroft-Langreth dynamic structure factors of liquid binary mixtures. We also provide expressions for the Bhatia-Thornton dynamic structure factors and, within these expressions, show how the model reproduces both the dynamic and the self-dynamic structure factors corresponding to a one-component system in the appropriate limits (pseudobinary system or zero concentration of one component). In particular we analyze the behavior of the concentration-concentration dynamic structure factor and longitudinal current, and their corresponding counterparts in the one-component limit, namely, the self dynamic structure factor and self longitudinal current. The results for several lithium alloys with different ordering tendencies are compared with computer simulations data, leading to a good qualitative agreement, and showing the natural appearance in the model of the fast sound phenomenon.Comment: 20 pages, 19 figures, submitted to PR

Geometrical resonance in spatiotemporal systems

We generalize the concept of geometrical resonance to perturbed sine-Gordon, Nonlinear Schr&#246;dinger and Complex Ginzburg-Landau equations. Using this theory we can control different dynamical patterns. For instance, we can stabilize breathers and oscillatory patterns of large amplitudes successfully avoiding chaos. On the other hand, this method can be used to suppress spatiotemporal chaos and turbulence in systems where these phenomena are already present. This method can be generalized to even more general spatiotemporal systems.Comment: 2 .epl files. Accepted for publication in Europhysics Letter

Exciton Gas Compression and Metallic Condensation in a Single Semiconductor Quantum Wire

We study the metal-insulator transition in individual self-assembled quantum wires and report optical evidences of metallic liquid condensation at low temperatures. Firstly, we observe that the temperature and power dependence of the single nanowire photoluminescence follow the evolution expected for an electron-hole liquid in one dimension. Secondly, we find novel spectral features that suggest that in this situation the expanding liquid condensate compresses the exciton gas in real space. Finally, we estimate the critical density and critical temperature of the phase transition diagram at $n_c\sim1\times10^5$ cm$^{-1}$ and $T_c\sim35$ K, respectively.Comment: 4 pages, 5 figure

Soliton tunneling with sub-barrier kinetic energies

We investigate (theoretically and numerically) the dynamics of a soliton moving in an asymmetrical potential well with a finite barrier. For large values of the width of the well, the width of the barrier and/or the height of the barrier, the soliton behaves classically. On the other hand, we obtain the conditions for the existence of soliton tunneling with sub-barrier kinetic energies. We apply these results to the study of soliton propagation in disordered systems.Comment: 6 eps figures. To appear in Physical Review E (Rapid Communications

From circular paths to elliptic orbits: A geometric approach to Kepler's motion

The hodograph, i.e. the path traced by a body in velocity space, was introduced by Hamilton in 1846 as an alternative for studying certain dynamical problems. The hodograph of the Kepler problem was then investigated and shown to be a circle, it was next used to investigate some other properties of the motion. We here propose a new method for tracing the hodograph and the corresponding configuration space orbit in Kepler's problem starting from the initial conditions given and trying to use no more than the methods of synthetic geometry in a sort of Newtonian approach. All of our geometric constructions require straight edge and compass only.Comment: 9 pages, 4 figure